Nontuberculous Mycobacteria (NTM) represent a group of mycobacteria other than Mycobacterium tuberculosis and Mycobacterium leprae. These bacteria are commonly found in nature and generally do not cause disease in humans, unless predisposing factors are present, such as a history of other lung diseases and immunodeficiency conditions like HIV-AIDS. The most common clinical manifestation of NTM infection is pulmonary disease, presenting symptoms resembling other chronic lung diseases, particularly tuberculosis. The prevalence of NTM infections is increasing worldwide. However, research on NTM prevalence in Indonesia remains extremely limited. This study is conducted to determine the prevalence of NTM infections in patients with clinical manifestations of tuberculosis and to characterize these patients. NTM infection identification is carried out through subject interviews, medical record data collection, and sputum sample testing using line probe assay (LPA).